Journal: Experimental and Therapeutic Medicine

Article Title: Preparation and properties of a novel carbon nanotubes/poly(vinyl alcohol)/epidermal growth factor composite biological dressing

doi: 10.3892/etm.2017.4752

Figure Lengend Snippet: Morphocytology of L929 mice fibroblasts after 48 h (magnification, ×100). (A) Control, (B) experimental group 1 and (C) experimental group 2.

Article Snippet: The L929 mouse fibroblast cell-line was provided by Nanjing KeyGen Biotech Co., Ltd.

Techniques: Control

Journal: Experimental and Therapeutic Medicine

Article Title: Preparation and properties of a novel carbon nanotubes/poly(vinyl alcohol)/epidermal growth factor composite biological dressing

doi: 10.3892/etm.2017.4752

Figure Lengend Snippet: Morphology of L929 mice fibroblasts after cultured in the dressing leaching solution and normal saline for a period of time, and continued to culture for 48 h. (magnification, ×40). (A) Cultured in normal saline; (B) cultured in dressing leaching solution for 15 min; (C) 1 h; (D) 9 h; (E) 12 h and (F) 48 h.

Article Snippet: The L929 mouse fibroblast cell-line was provided by Nanjing KeyGen Biotech Co., Ltd.

Techniques: Cell Culture, Saline

Journal: International Journal of Molecular Sciences

Article Title: Mts1 (S100A4) and Its Peptide Demonstrate Cytotoxic Activity in Complex with Tag7 (PGLYRP1) Peptide

doi: 10.3390/ijms25126633

Figure Lengend Snippet: ( a ) Cytotoxic activity of the 17.1-Mts1 complex (1 nM) on L929, K562, HEK 293T, and HL-60 cells in the presence of an antibody to TNFR1 (1:100, 24 h). ( b ) Cytotoxic activity of Mts1 complexes with peptides 17.1, 17.1A, and 17.1B on L929 cells (24 h). ( c ) Concentration dependence of the cytotoxic activity of the 17.1-Mts1 complex on L929 cells (24 h). n = 5 for each point (* p -value < 0.05).

Article Snippet: Experiments were performed with the L929 mouse fibroblast cell line, which was cultured, respectively, in DMEM (Himedia Laboratories Private Limited, Maharashtra, India) with 2 mM L-glutamine, 10% FCS (Cytiva LivescienceTM, Marlborough, MA, USA), and antibiotics (penicillin and streptomycin) (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C in an atmosphere containing 5% CO 2 .

Techniques: Activity Assay, Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Mts1 (S100A4) and Its Peptide Demonstrate Cytotoxic Activity in Complex with Tag7 (PGLYRP1) Peptide

doi: 10.3390/ijms25126633

Figure Lengend Snippet: Cytotoxic activity of the 17.1-Mts1 complex on L929 cells after 3 ( a ) and 24 ( c ) hours in the presence of caspase 3 and 8 inhibitors and necrostatin1. n = 5 for each point, (* p -value < 0.05). ( b ) Western blot with antibodies to caspase 3 from cell lysates 1 h after 17.1-Mts1 addition (left) and control cells (right). ( d ) Western blot with antibodies to phospho-MLKL, RIP3, and phospho-RIP3 from cell lysates 1 h after addition of 17.1-Mts1 ( left ) and control cells ( right ).

Article Snippet: Experiments were performed with the L929 mouse fibroblast cell line, which was cultured, respectively, in DMEM (Himedia Laboratories Private Limited, Maharashtra, India) with 2 mM L-glutamine, 10% FCS (Cytiva LivescienceTM, Marlborough, MA, USA), and antibiotics (penicillin and streptomycin) (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C in an atmosphere containing 5% CO 2 .

Techniques: Activity Assay, Western Blot, Control

Journal: International Journal of Molecular Sciences

Article Title: Mts1 (S100A4) and Its Peptide Demonstrate Cytotoxic Activity in Complex with Tag7 (PGLYRP1) Peptide

doi: 10.3390/ijms25126633

Figure Lengend Snippet: Cytotoxic activity of the 17.1-M7 complex on L929 cells after 3 ( a ) and 20 ( b ) hours in the presence of caspase 3 and 8 inhibitors, necrostatin1, EGTA, calpains and cathepsins inhibitors, and antioxidant ionol. n = 5 for each point, (* p -value < 0.05).

Article Snippet: Experiments were performed with the L929 mouse fibroblast cell line, which was cultured, respectively, in DMEM (Himedia Laboratories Private Limited, Maharashtra, India) with 2 mM L-glutamine, 10% FCS (Cytiva LivescienceTM, Marlborough, MA, USA), and antibiotics (penicillin and streptomycin) (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C in an atmosphere containing 5% CO 2 .

Techniques: Activity Assay

Journal: Polymers

Article Title: Antibacterial Chitosan Nanofiber Thin Films with Bacitracin Zinc Salt

doi: 10.3390/polym13071104

Figure Lengend Snippet: The cell viability of L929 fibroblasts after incubation with the thin films for 24 h (** p < 0.01).

Article Snippet: The cytotoxicity of the films was estimated from the viability of L929 fibroblasts (KAC Co., Ltd., Kyoto, Japan).

Techniques: Incubation

Journal: Annals of Medicine and Surgery

Article Title: Effects of heated tobacco products and conventional cigarettes on dental implant wound healing: experimental research

doi: 10.1097/MS9.0000000000000367

Figure Lengend Snippet: (A) Scratch wound-healing assay. Images from scratch assay experiments at different time-points (×40 magnification). Migrated cells are shown in red. (B) Number of L929 cell migration into a cell-free area. The number of L929 cells decreased on exposure to cigarette smoke extract from HTPs and CCs ( n =3). CCs, conventional cigarettes; HTPs, heated tobacco products.

Article Snippet: The L929 mouse fibroblast cell line was purchased from Sumitomo Pharma and cultured in MEM containing 5% foetal bovine serum, 100 U/ml penicillin, and 100 g/ml streptomycin (Thermo Fisher Scientific).

Techniques: Wound Healing Assay, Migration

Journal: Pharmaceutics

Article Title: What We Learned about the Feasibility of Gene Electrotransfer for Vaccination on a Model of COVID-19 Vaccine

doi: 10.3390/pharmaceutics15071981

Figure Lengend Snippet: Expression of S and N 2 days after in vitro GET to L929 fibroblasts and C2C12 myoblasts: ( a ) S mRNA expression; ( b ) N mRNA expression; ( c ) concentration of S protein in cell lysate; and ( d ) concentration of N protein in cell lysate. Graph bars represent the mean with standard error of the mean (SEM) of three independent experiments with 3 technical replicates. Ctrl, non-transfected cells (nuclease-free water + EP); N.D., not detected. One-way ANOVA: *, p ≤ 0.05; and ***, p ≤ 0.001.

Article Snippet: The C2C12 mouse myoblast cell line (American Type Culture Collection, ATCC, Manassas, VA, USA; ATCC ® CRL-1772™) and the L929 mouse fibroblast cell line (Merck, Kenilworth, NJ, USA) were cultured in advanced DMEM medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 5% fetal bovine serum (FBS, Gibco), GlutaMAX (100×, Gibco), and penicillin–streptomycin (100×, Sigma-Aldrich, Merck, Darmstadt, Germany) grown in a 5% CO 2 humidified atmosphere at 37 °C.

Techniques: Expressing, In Vitro, Concentration Assay, Transfection

Journal: Virology

Article Title: Lethal murine infection model for human respiratory disease-associated Pteropine orthoreovirus

doi: 10.1016/j.virol.2017.10.021

Figure Lengend Snippet: Neutralization activity (mean (95% CI)) of anti-PRV serum against Reoviridae viruses.

Article Snippet: Mouse fibroblast L929, lung adenocarcinoma A549, and rhesus monkey kidney MA104 cell lines were grown in Dulbecco's modified Eagle's medium (DMEM) (Nacalai Tesque) supplemented with 5% fetal bovine serum (Gibco).

Techniques: Neutralization, Activity Assay